عنوان: Sperm-Mediated Gene Transfer Concepts and Controversies
قیمت: 6,900 تومان
As a young researcher in an established transgenic laboratory at Edinburgh University in 1989, I was struck by the effects of the publication in the journal Cell of a paper reporting that mammalian sperm could readily act as vectors for foreign DNA. Almost overnight, attention in our laboratory switched from established methods of transgenesis towards this exciting new prospect, and immediate efforts were made to replicate the Cell work. The talk at the time was that all the paraphernalia, training and expense of pronuclear microinjection (the standard approach) would be rendered redundant by this disruptive new technology, known as sperm-mediated gene transfer (SMGT). In the event, the original Cell work could not be replicated, either in our laboratory or in a number of laboratories worldwide that were similarly attempting to make SMGT work. This failure was experienced by many in the field of animal transgenesis as something of a body-blow. Researchers were genuinely very disappointed that SMGT manifestly did not deliver on its initial promise. A backlash followed, with a substantial amount of skepicism being directed towards both the Cell paper and also towards the fundamental biological notion that sperm could ever be expected to be able to act as transgene vectors.